Genetic diversity of Fusarium oxysporum f.sp. niveum responsible of watermelon Fusarium wilt in Tunisia and Spain
Abstract
Fusarium wilt is a serious disease, caused by Fusarium oxysporum f. sp. niveum (FON) and responsible of significant yield losses in watermelon crop in Tunisia. Thirty-nine FON isolates were collected from different infected watermelon fields in Tunisia and were identified on the basis of morphological features and by using one pair of species-specific primers Fn-1/Fn-2. Genetic diversity of the twenty-six FON isolates, originated from Spain and Tunisia, was studied by applying Inter simple sequence repeat genotyping. PCR amplification appears to be efficient to identify FON isolates amplifying only a single PCR band of approximately 800 bp. The RAMS study using two, bi and four trinucleotide microsatellites primers, showed a different genetic similarity degree among FON isolates. Seventy-one bands were amplified by four ISSR primer combinations. Diversity in the banding patterns obtained by DNA fingerprinting was always >50% and allowed us to distinguish all the isolates tested, according to number and size of the fragments, which ranged from 300 to 2800 bp. The genetic similarity values are comprised between 8 and 97%. UPGMA grouped the 45 genotypes into six main clusters at a similarity index value above 0.5, showing a relative genetic homogeneity and no correlation has been found among FON isolates and their origins. The most abundant Cluster VI comprising thirty-nine FON isolates.
Metrics
Published
How to Cite
Issue
Section
License
Authors who publish with Journal of Phytopathology and Disease Management agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under the Creative Commons Attribution-Non Commercial License (CC BY-NC). This allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Archives of Agricultural Sciences Journal is an Open Access Journal, and articles published are distributed under the terms of the Creative Commons Attribution-Non Commercial License (CC BY-NC). Readers may copy, distribute, and display the work for non commercial purposes with the proper citation of the original work. However, the journal retains the right to exploit subsidiary rights on behalf of the authors.
- Authors are able to enter into separate, additional contractural arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process with full disclosure to the journal, as it can lead to productive exchanges, as well as earlier and greater citation of published work. Following publication in Archives of Agricultural Sciences Journal, the author should update the repository, and include a citation and link to the published work.
Click here for more information on Licensing policy