Chia charcoal rot disease and its management using certain bio-agents in Egypt

Authors

  • Madian M. Mergawy Central laboratory of Organic Agriculture, Agricultural Research Center (ARC), Giza, Egypt
  • Mahmoud M. H. Hassanin Ornamental, Medicinal and Aromatic Plant Disease Department, Plant Pathology Research Institute, Agricultural Research Center (ARC), Giza, Egypt
  • Sarah A. A. Ahmed Central laboratory of Organic Agriculture, Agricultural Research Center (ARC), Giza, Egypt
  • Abdallah A. M. Ali

DOI:

https://doi.org/10.5281/zenodo.10077764

Keywords:

chia, charcoal rot, Macrophomina phaseolina, Trichoderma asperellum, Streptomyces rochei

Abstract

The current study was performed to control the charcoal stem rot disease caused by Macrophomina phaseolina in chia plants (Salvia hispanica L.). Macrophomina phaseolina was morphologically identified as the causal pathogen of charcoal stem rot on naturally infected chia plants showing typical symptoms of disease and obtained from Fayoum, Giza, and Menoufia governorates, Egypt in the 2021 cultivation season. Additionally, the molecular characterization of the causal pathogen revealed 99.65-100% identity with several isolates of the same species. The isolates obtained from all governorates under study resulted in pre- and post-emergence damping-off with significant variations. However, M. phaseolina isolated from Fayoum governorate recorded the highest percentage of charcoal stem rot. Moreover, the filtrate of the same isolate caused the highest percentages of wilted seedlings. The presence of yeast, amonium chloride and urea as nitrogen sources resulted in the loss of M. phaseolina mycelial color. The pigmented isolate of M. phaseolina (Fayoum isolate) exhibited a high virulence to chia plants in greenhouse compared to the non-pigmented one. The two bio-agents; Trichoderma asperellum and Streptomyces rochei, isolated from the rhizosphere soil of healthy chia plants, significantly inhibited M. phaseolina fungal growth in comparison with the control in vitro. In greenhouse experiment, the fungicide Tricyclazole was the most efficient application for reducing the incidence of disease as well as increasing the plant growth measurements, i.e., number of spikes per plant, number of branches and plant height, followed by the biocide New-Actino. The combined use of the bio-agents T. asperellum and S. rochei was greatly efficient in both decreasing the disease incidence and improving the plant growth parameters compared to individual use of each. The current study indicated the potential use of the biocide New-Actino, T. asperellum and S. rochei as fungicidal alternatives for controlling chia charcoal rot disease.

Metrics

Metrics Loading ...

Downloads

Published

2022-12-01

How to Cite

Mergawy, M., Hassanin, M., Ahmed, S., & Ali, A. (2022). Chia charcoal rot disease and its management using certain bio-agents in Egypt. Journal of Phytopathology and Disease Management, 9(1), 14–31. https://doi.org/10.5281/zenodo.10077764

Issue

Vol. (9), Issue (1)

Section

Research Articles

URN

https://urn.issn.org/urn:issn:3009-6111urn:issn:3009-6111PPDJ.v9i1.245

ARK

https://n2t.net/ark:/24629/PPDJ.v9i1.245

License

Copyright (c) 2022 Journal of Phytopathology and Disease Management

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with Journal of Phytopathology and Disease Management agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under the Creative Commons Attribution-Non Commercial License (CC BY-NC). This allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  3. Archives of Agricultural Sciences Journal is an Open Access Journal, and articles published are distributed under the terms of the Creative Commons Attribution-Non Commercial License (CC BY-NC). Readers may copy, distribute, and display the work for non commercial purposes with the proper citation of the original work. However, the journal retains the right to exploit subsidiary rights on behalf of the authors.
  4. Authors are able to enter into separate, additional contractural arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  1. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process with full disclosure to the journal, as it can lead to productive exchanges, as well as earlier and greater citation of published work. Following publication in Archives of Agricultural Sciences Journal, the author should update the repository, and include a citation and link to the published work.

      Click here for more information on Licensing policy