Morpho-molecular identification and management of Erysiphe heraclei causing dill powdery mildew using a biocide, essential oils, and organic acids

Authors

  • Mahmoud M. H. Hassanin Plant Pathology Research Institute, Agricultural Research Centre, Giza, Egypt
  • Abdallah A. M. Ali Plant Pathology Department, Faculty of Agriculture and Natural Resources, Aswan University, Aswan, Egypt
  • Heba Yousef Plant Pathology Research Institute, Agricultural Research Centre, Giza, Egypt
  • Madian M. Mergawy Central Laboratory of Organic Agriculture (CLOA), Agricultural Research Center (ARC), Giza, Egypt

DOI:

https://doi.org/10.5281/zenodo.10076646

Keywords:

dill, Erysiphe heraclei, powdery mildew, Bio-Cur F, thyme essential oil, salicylic acid

Abstract

This study aimed to evaluate the efficacy of Bio-Cure F, essential oils of thyme and clove, ascorbic and salicylic acids in controlling dill powdery mildew under greenhouse conditions. The causal pathogen of powdery mildew was isolated from infected dill plants showing symptoms of the disease during a survey in Fayoum governorate, Egypt, in 2022. Erysiphe heraclei was morphologically characterized using a light microscope as the causal pathogen of powdery mildew in infected dill plants. The nucleotide sequence of the internal transcribed spacer (ITS) of the causal pathogen DNA was deposited in GenBank (Accession No. OP999071.1), which showed 98-100% similarity with accession numbers of E. heraclei isolates obtained from the NCBI database. Under greenhouse conditions, dill plants were sprayed with biocide (Bio-Cure F), elicitors (ascorbic and salicylic acids), and essential oil emulsions of clove and thyme three times after inoculation with E. heraclei. The results showed that Bio-Cure F was the treatment that showed the highest efficacy in reducing both disease severity and incidence percentage, followed by thyme essential oil. The highest percentages of increase in dill plant height (cm) as well as branches/plant were obtained by Bio-Cure F treatment compared to the control, followed by salicylic acid treatment. The results suggest that Bio-Cure F, salicylic acid, and thyme oil emulsion could be used to control dill powdery mildew.

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Published

2023-07-01

How to Cite

Hassanin, M., Ali, A., Yousef, H., & Mergawy, M. (2023). Morpho-molecular identification and management of Erysiphe heraclei causing dill powdery mildew using a biocide, essential oils, and organic acids. Journal of Phytopathology and Disease Management, 10(1), 14–25. https://doi.org/10.5281/zenodo.10076646

Issue

Vol. (10), Issue (1)

Section

Research Articles

URN

https://urn.issn.org/urn:issn:3009-6111urn:issn:3009-6111PPDJ.v10i1.243

ARK

https://n2t.net/ark:/24629/PPDJ.v10i1.243

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