Selection of compost-derived actinomycetes with plant-growth promoting and tomato stem rot biocontrol potentialities


  • Fakher Ayed 1National Agronomic Institute of Tunisia, 1082 Tunis, University of Carthage, Tunisia 2Technical Centre of Organic Agriculture, 4042 Chott-Meriam, Sousse, Tunisia 3UR13AGR09-Integrated Horticultural Production in the Tunisian Centre-East, Regional Research Centre on Horticulture and Organic Agriculture, University of Sousse, 4042, Chott-Meriem, Tunisia
  • Rania Aydi-Ben Abdallah UR13AGR09-Integrated Horticultural Production in the Tunisian Centre-East, Regional Research Centre on Horticulture and Organic Agriculture, University of Sousse, 4042, Chott-Meriem, Tunisia
  • Hayfa Jabnoun-Khiareddine UR13AGR09-Integrated Horticultural Production in the Tunisian Centre-East, Regional Research Centre on Horticulture and Organic Agriculture, University of Sousse, 4042, Chott-Meriem, Tunisia
  • Mejda Daami-Remadi UR13AGR09-Integrated Horticultural Production in the Tunisian Centre-East, Regional Research Centre on Horticulture and Organic Agriculture, University of Sousse, 4042, Chott-Meriem, Tunisia


actinobacteria, antifungal activity, growth enhancement, Sclerotium rolfsii, Solanum lycopersicum


Seventeen actinomycetes isolates, recovered from 2 composts, were screened for their ability to promote the growth of tomato seedlings and to suppress stem rot disease caused by Sclerotium rolfsii. Tomato cv. Rio Grande seedlings inoculated with S. rolfsii and treated with A2-3, A3-3, A4-3, A5-3, A8-3, A9-3, A1-4, A2-4, A3-4, A4-4, A6-4, and A10-4 actinobacterial isolates showed 23.3-70% less disease severity than the inoculated and untreated controls. A3-3, A2-4, and A4-4 based treatments applied to S. rolfsii-infected tomato seedlings had significantly enhanced all growth parameters as compared to control. The recorded increments were estimated at 35.52-66.6% for height, 37.4-53.4% for the stem diameter, 38.5-95.6% for the aerial part dry weight, and 81.8-151% for the root dry weight. Treatments with A3-3 and A4-4 isolates had increased the majority of tomato growth parameters by 15.8-56.5% over the pathogen-free control. Tomato seedlings treated with A4-3 and A1-4 isolates showed between 35.2-22.8% and 42.3-43.3% higher aerial part dry weight and root dry weight, respectively, as compared to pathogen-free and untreated control. This investigation demonstrated that the tested composts can be explored as potential sources for the isolation of actinomycetes acting as biocontrol and bio-fertilizing agents.


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Alfano G, Lustrato G, Lima G, Vitullo D, Ranalli G, 2011. Characterization of composted olive mill wastes to predict potential plant disease suppressiveness. Biological Control 58(3): 99–207.

Aouar L, Lerat S, Ouffroukh A, Boulahrouf A, Beaulieu C, 2012. Taxonomic identification of rhizospheric actinobacteria isolated from Algerian semi-arid soil exhibiting antagonistic activities against plant fungal pathogens. Canadian Journal of Plant Pathology 34: 165–176.

Anusree T, Bhai RS, 2017. Rhizosphere Actinobacteria for combating Phytophthora capsici and Sclerotium rolfsii, the major soil borne pathogens of black pepper (Piper nigrum L). Biological Control 109: 1–13.

Aouar L, Lerat S, Ouffroukh A, Boulahrouf A, Beaulieu C, 2012. Taxonomic identification of rhizospheric actinobacteria isolated from Algerian semi-arid soil exhibiting antagonistic activities against plant fungal pathogens. Canadian Journal of Plant Pathology 34(2): 165–176.

Aycock R, 1966. Stem rot and other diseases caused by Sclerotium rolfsii or the status of Rolf’s fungus after 70 years. Technical Bulletin/North Carolina Agricultural Experiment Station 174: 136–202.

Aydi-Ben Abdallah R, Jabnoun-Khiareddine H, Nefzi A, Ayed F, Daami-Remadi M, 2018. Endophytic bacteria from Solanum nigrum with plant growth-promoting and Fusarium wilt-suppressive abilities in tomato. Tunisian Journal of Plant Protection 13(2): 157–182.

Aydi-Ben Abdallah R, Jabnoun-Khiareddine H, Daami-Remadi M, 2020. Variation in the composition of potato plants depending on cropping season, cultivar type, and plant development stage. International Journal of Agriculture, Environment and Food Science 4(3): 319–333.

Ayed F, Jabnoun-Khiareddine H, Aydi-Ben-Abdallah R, Daami-Remadi M, 2018a. Effect of temperatures and culture media on Sclerotium rolfsii mycelial growth, sclerotial formation and germination. Journal of Plant Pathology and Microbiology 09: 429.

Ayed F., Jabnoun-Khiareddine H. and Daami-Remadi M, 2018b. Valorisation du compost comme moyen de lutte contre Sclerotium rolfsii agent responsable de la pourriture des tiges. Actes des 9èmes Journées Internationales sur la Valorisation des Bioressources à Monastir, Tunisia, C.O. 48.

Ayed F., Jabnoun-Khiareddine H., Aydi Ben Abdallah R. and Daami-Remadi M, 2018c. Sclerotium collar rot suppression and tomato growth promotion achieved using different compost teas. Proceedings of the fifth International Conference on Sustainable Agriculture and Environment, Hammamet, Tunisia, pp. 61.

Bhatti AA, Haq S, Bhat RA, 2017. Actinomycetes benefaction role in soil and plant health. Microbial Pathogenesis 111: 458–467.

Bibha D, Githanjali N, Lora P, Volker SB, 2017. Diversity of free living nitrogen fixing Streptomyces in soils of the badlands of South Dakota. Microbiological Research 195: 31–39.

Bohacz J, 2018. Microbial strategies and biochemical activity during lignocellulosic waste composting in relation to the occurring biothermal phases. Journal of Environmental Management 206: 1052–1062.

Coelho L, Reis M, Guerrero C, Dionísio L, 2020. Use of organic composts to suppress bentgrass diseases in Agrostis stolonifera. Biological control 141: 104154.

Coventry E, Noble R, Mead A, Marin F, Perez J, Whipps J, 2006. Allium white rot suppression with composts and Trichoderma viridae in relation to sclerotia viability. Phytopathology 96(9): 1009–1020.

Daami-Remadi M, Jabnoun-Khiareddine H, Sdiri A, El Mahjoub M, 2012. Comparative reaction of potato cultivars to Sclerotium rolfsii assessed by stem rot and tuber decay severity. Pest Technology 6(Special Issue 1): 54–59.

Dandan H, Lanying W, Yanping L, 2018. Isolation, identification, and the growth promoting effects of two antagonistic actinomycete strains from the rhizosphere of Mikania micrantha Kunth. Microbiological Research 208: 1–11.

De Corato U, 2020. Disease-suppressive compost enhances natural soil suppressiveness against soil-borne plant pathogens: A critical review. Rhizosphere 130: 100192.

De Curtis F, Lima G, Vitullo D, De Cicco V, 2010. Biocontrol of Rhizoctonia solani and Sclerotium rolfsii on tomato by delivering antagonistic bacteria through a drip irrigation system. Crop Protection 29: 663–670.

Errakhi R, Bouteau F, Lebrihi A, Barakate M, 2007. Evidences of biological control capacities of Streptomyces spp. against Sclerotium rolfsii responsible for damping-off disease in sugar beet (Beta vulgaris L.). World Journal of Microbiology and Biotechnology 23: 1503–1509.

Fery RL, Dukes PD, 2002. Southern Blight (Sclerotium rolfsii Sacc.) of Cowpea: Yield-loss estimates and sources of resistance. Crop Protection 21: 403–408.

Getha K, Vikineswary S, 2002. Antagonistic effects of Streptomyces violaceusniger strain G10 on Fusarium oxysporum f.sp. cubense race 4: indirect evidence for the role of antibiosis in the antagonistic process. Journal of Industrial Microbiology & Biotechnology 28(6): 303–310.

Golinska P, Dahm H, 2013. Antagonistic properties of Streptomyces isolated from forest soils against fungal pathogens of pine seedlings. Dendrobiology 69: 87–97.

Goodfellow M, Simpson KE, 1987. Ecology of Streptomycetes. Frontiers in Applied Microbiology 2: 97–125.

Gopalakrishnan S, Vadlamudi S, Bandikinda P, Sathya A, Vijayabharathi R, Rupela O, Kudapa H, Katta K, Varshney RK, 2014. Evaluation of Streptomyces strains isolated from herbal vermicompostfor their plant growth-promotion traits in rice. Microbiological Research 169: 40–48.

Goudjal Y, Toumatia O, Sabaou N, Barakate M, Mathieu F, Zitouni A, 2013. Endophytic actinomycetes from spontaneous plants of Algerian Sahara: indole-3-acetic acid production and tomato plants growth promoting activity. World Journal of Microbiology and Biotechnology 29: 1821–1829.

Hadar Y, Papadopoulou KK, 2012. Suppressive composts: microbial ecology links between abiotic -environments and healthy plants. Annual Review of Phytopathology 50: 133–153.

Hamdali H, Hafidi M, Virolle MJ, Ouhdouch Y, 2008. Rock phosphate-solubilizing Actinomycetes: screening for plant growth-promoting activities. World Journal of Microbiology and Biotechnology 24: 2565–2575.

Himaman W, Thamchaipenet A, Pathomaree W, Duangmal K, 2016. Actinomycetes from Eucalyptus and their biological activities for controlling Eucalyptus leaf and shoot blight. Microbiological Research 188-189: 42–52.

Jayamurthy H, Valappil K, Dastagar SG, Pandey A, 2014. Anti-fungal potentials of extracellular metabolites of Western Ghats isolated Streptomyces sp. NII 1006 against moulds and yeasts. Indian Journal of Experimental Biology 52: 1138–1146.

Jose PA, Jha B, 2016. New dimensions of research on Actinomycetes: quest for next generation antibiotics. Frontiers in Microbiology 7: 1295.

Joshi D, Hooda KS, Bhatt JC, Mina BL, Gupta HS, 2009. Suppressive effects of composts on soil-borne and foliar diseases of French bean in the field in the western Indian Himalayas. Crop Protection 28: 608–615.

Kator L, Hosea ZY, Oche OD, 2015. Sclerotium rolfsii; Causative organism of Southern blight, stem rot, white mold and sclerotia rot disease. Annals of Biological Research 6: 78–89.

Khanna M, Solanki R, Lal R, 2011. Selective isolation of rare actinomycetes producing novel antimicrobial compounds. International Journal of Advanced Biotechnology and Research 2: 357–375.

Kobayashi YO, Kobayashi A, Maeda M, Takenaka S, 2012. Isolation of antagonistic Streptomyces sp.: against a potato scab pathogen from a field cultivated with wild oat. Journal of General Plant Pathology 78: 62–72.

Larkin RP, Tavantzis S, 2013. Use of biocontrol organisms and compost amendments for improved control of soilborne diseases and increased potato production. American Journal of Potato Research 90: 261–270.

Manici LM, Caputo F, Babini V, 2004. Effect of green manure on Pythium sp. population and microbial communities in intensive cropping systems. Plant Soil 263: 133–142.

Martin SCCG, 2015. Enhancing soil suppressiveness using compost and compost tea. In: Meghvansi MK, Varma A, eds. Organic amendments and soil suppressiveness in plant disease management. Soil Biology, Volume 46, Springer International Publishing, Switzerland, pp. 25–49.

Mingma R, Pathom-aree W, Trakulnaleamsai S, Thamchaipenet A, Duangmal K, 2014. Isolation of rhizospheric and roots endophytic actinomycetes from Leguminosae plant and their activities to inhibit soybean pathogen Xanthomonas campestris pv. glycine. World Journal of Microbiology & Biotechnology 30: 271–280.

Nurkanto A, Julistiono H, 2014. Screening and study of antifungal activity of leaf litter actinomycetes isolated from Ternate island, Indonesia. Asian Pacific Journal of Tropical Medicine 7: S238–S243.

Palaniyandi SA, Yang SH, Zhang L, Suh JW, 2013. Effects of actinobacteria on plant disease suppression and growth promotion. Applied Microbiology and Biotechnology 97: 9621–9636.

Palla MS, Guntuku GS, Muthyala MKK, Pingali S, Sahu PS, 2018. Isolation and molecular characterization of antifungal metabolite producing actinomycete from mangrove soil. Beni-Suef University Journal of Basic and Applied Sciences 7: 250–256.

Pane C, Piccolo A, Spaccini R, Celano G, Villecco D, Zaccardelli M, 2013. Agricultural waste-based composts exhibiting suppressivity to diseases caused by the phytopathogenic soil-borne fungi Rhizoctonia solani and Sclerotinia minor. Applied Soil Ecology 65: 43–51.

Pane C, Spaccini R, Piccolo A, Celano G, Zaccardelli M, 2019. Disease suppressiveness of agricultural green-waste composts as related to chemical and bio-based properties shaped by different on-farm composting methods. Biological Control 137: 104026.

Patil HJ, Srivastava AK, Kumar S, Chaudhari BL, Arora DK, 2010. Selective isolation, evaluation and characterization of antagonistic actinomycetes against Rhizoctonia solani. World Journal of Microbiology and Biotechnology 26(12): 2163–2170.

Peralta KD, Araya T, Valenzuela S, Sossa K, Martínez M, Cortés HP, Sanfuentes E, 2012. Production of phytohormones, siderophores and population fluctuation of two root-promoting rhizobacteria in Eucalyptus globulus cuttings. World Journal of Microbiology and Biotechnology 28: 2003–2014.

Punja ZK, 1985. The biology, ecology and control of Sclerotium rolfsii. Annual Review of Phytopathology 23: 97–127.

Qin S, Xing K, Jiang JH, Xu LH, Li WJ, 2011. Biodiversity, bioactive natural products and biotechnological potential of plant-associated endophytic actinobacteria. Applied Microbiology and Biotechnology 89: 457–473.

Salla TD, da Silva TR, Astarita LV, Santarém ER, 2014. Streptomyces rhizobacteria modulate the secondary metabolism of Eucalyptus plants. Plant Physiology and Biochemistry 85: 14–20.

Scotti R, Pane C, Spaccini R, Palese AM, Piccolo A, Celano G, Zaccardelli M, 2016. On-farm compost: a useful tool to improve soil quality under intensive farming systems. Applied Soil Ecology 107: 13–23.

Singh SP, Singh HB, Singh DK, 2013. Trichoderma harzianum and Pseudomonas sp. mediated management of Sclerotium rolfsii rot in tomato (Lycopersicon esculentum Mill.). Life Sciences 8: 801–804.

Sridharan AP, Sugitha T, Karthikeyan G, Sivakumar U, 2020. Comprehensive profiling of the VOCs of Trichoderma longibrachiatum EF5 while interacting with Sclerotium rolfsii and Macrophomina phaseolina. Microbiological Research 236: 126436.

Srivastava S, Patel JS, Singh HB, Sinha A, Sarma BK, 2015. Streptomyces rocheiSM3 induces stress tolerance in chickpea against Sclerotinia sclerotiorum and NaCl. Journal of Phytopathology 163: 583–592.

Stavi I, Bel G, Zaady E, 2016. Soil functions and ecosystem services in conventional, conservation, and integrated agricultural systems. A review. Agronomy for Sustainable Development 36: 32.

Sun S, Sun F, Deng D, Zhu X, Duan C, Zhu Z, 2020. First report of southern blight of mung bean caused by Sclerotium rolfsii in China. Crop Protection 130: 105055.

Tubeileh AM, Stephenson GT, 2020. Soil amendment by composted plant wastes reduces the Verticillium dahliae abundance and changes soil chemical properties in a bell pepper cropping system. Current Plant Biology 22: 100148.

Yuan WM, Crawford DL, 1995. Characterization of Streptomyces lydicus WYEC108 as a potential biocontrol agent against fungal root and seed rots. Applied and Environmental Microbiology 61: 3119–3128.



How to Cite

Ayed, F., Aydi-Ben Abdallah, R., Jabnoun-Khiareddine, H., & Daami-Remadi, M. (2021). Selection of compost-derived actinomycetes with plant-growth promoting and tomato stem rot biocontrol potentialities. Journal of Phytopathology and Disease Management, 8(1), 79–91. Retrieved from



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