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1. Introduction
For many years, varietal identification of
bee honeys has been a research subject in
many scientific centers (Bogdanov,
1999). Using pollen analysis to assess
quality and botanical origin of honey was
discussed by Lukacs (1997). Quality
control methods have been found to be
able to classify honeys from different
geographical regions. Bee honeys were
considered monofloral honey whenever
the dominant pollen type was found to be
over 45% of total pollen grains in the
tested honey (Amaral et al., 2003). Also,
both pollen identification and count have
been used to determine the authentication
of honey according to the floral type
(Serrano et al., 2004). Results of Wen et
al. (1995) indicated that, about 30% of
samples were adulterated with sugar
syrup or other products. Due to its
simplicity, pollen analysis was used
extensively to identify different types of
honey samples from different botanical
origins in different countries (Devillers et
al., 2004; Marini et al.,
2004; Cordella et
al., 2002; 2003). Pollen spectra of honey
were influenced by time of the year and
location (Ponnuchamy et al., 2014). In
Egypt, the qualitative pollen analysis of
honeys was done by many authors (El-
Metwally, 2015; Rateb, 2005; Nour et al.,
1991; Nour, 1988). Pollen analysis of
sixty Egyptian honey samples was done
by Nour (1988); he found that the main
pollen sources of Egyptian honeys were
clover (
Trifolium alexandrinum
L.),
eucalyptus (
Eucalyptus
spp.),
Citrus
sp.
date palm (
Phoenix dactylifera
L.), maize
(
Zea mays
L.), sunflower
(
Helianthus
annus
L.) and faba bean
(
Vicia fabae
L.).
Cotton pollens were estimated at less than
1% of the total pollen found in market
cotton honey samples. Rateb (2005)
identified fifteen pollen types in honey
samples from Assiut region, most of them
(80%) from necteriferous plants. The
main polleneferous plants of pollen
spectrum in the studied honeys were
Zea
mays, Phoenix dactylifera
and
Casuarina
equistifolia
L. Also, the same author
reported that pollen density of honey
collected by Egyptian honey bee race
was 9.6, 13.6, 5.1 and 16.1 times more
than those collected by Italian,
Caucaseca, Carniolan hybrid and
Carniolan honey bee races, respectively.
Furthermore, pollen density varied and
depended on the collection locality.
Morever, Egyptian bee honey samples
were examined by El-Metwally (2015)
and fourteen types of pollen were
classified. Clover pollen was found in
high percentage (30.2%) while date palm
and umbliferus pollen were recorded in
considerable percentages as 13.20 and
9.39%, respectively. The present work
aims to evaluate and classify some
commercial honeys (trademark) produced
in different localities in Egypt according
to their botanical origin.
2. Materials and methods
Laboratory works of the present
investigation were carried out at Plant
Protection Department, Faculty of
Agriculture, Assiut University, Egypt
during 2017 season.
2.1 Collection of honey samples
A total of 16 commercial honey samples
with trademark were collected from
markets in different regions of Egypt
during 2017 season (Table 1) for
evaluation and classification.
2.2 Pollen content determination
The method recommended by the
International Commission of Bee Botany
(ICBB) for pollen analysis was followed