Molecular characterization and biological control of some rice seed-borne fungal pathogens
Abstract
Seed-borne fungi cause enormous losses in rice production in Egypt. Ten different fungal species were isolated from four rice seed cultivars (Giza 177, Giza 179, Sakha 101 and Sakha 106) showing grain discoloration symptoms. Ten fungal species were initially identified as Fusarium spp. and Bipolaris spp. based on conidial morphology, colony appearance, pigmentation and growth rate. Molecular identification of these fungal isolates via PCR utilizing Internal transcribed spacer (ITS) region universal primers was carried out. ITS region was amplified to confirm the species identification. DNA sequence of PCR products and analysis via BLAST and data of the Genbank showed that four isolates belonging to F. graminearum, four isolates belonging to F. verticilliodies and two isolates identified as Bipolaris oryzae. The phylogenetic tree revealed different levels of molecular variation among the fungal species isolates compared to the international isolates deposited in the Genbank. Two biological control isolates of T. harzianum (Tr1 and Tr2) were used against F. graminearum, F. verticilliodies and B. oryzae isolates. The highest growth inhibition was exhibited by both T. harzianum isolates against F. graminearum (76.77% and 76.74% respectively), followed by F. verticilliodies. The least growth inhibition was observed on Bipolaris oryzae using Tr2 and Tr1 isolates (53.33% and 50.0% respectively). Moreover the naturally infected rice grains treated with both isolates Tr1 and Tr2 showed 100% inhibition of fungal pathogens associated with rice grains compared to untraded naturally infected grains.
Metrics
Published
How to Cite
Issue
Section
License
Authors who publish with Journal of Phytopathology and Disease Management agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under the Creative Commons Attribution-Non Commercial License (CC BY-NC). This allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Archives of Agricultural Sciences Journal is an Open Access Journal, and articles published are distributed under the terms of the Creative Commons Attribution-Non Commercial License (CC BY-NC). Readers may copy, distribute, and display the work for non commercial purposes with the proper citation of the original work. However, the journal retains the right to exploit subsidiary rights on behalf of the authors.
- Authors are able to enter into separate, additional contractural arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process with full disclosure to the journal, as it can lead to productive exchanges, as well as earlier and greater citation of published work. Following publication in Archives of Agricultural Sciences Journal, the author should update the repository, and include a citation and link to the published work.
Click here for more information on Licensing policy
Print ISSN: 3009-6111.png)
