Antifungal activity of different size controlled stable silver nanoparticles biosynthesized by the endophytic fungus Aspergillus terreus
Keywords: silver nanoparticles, size control, pH, biosynthesis, Aspergillus terreus, antifungal activity.
AbstractSilver nanoparticles (AgNPs) synthesized by using the aqueous extract of the endophytic fungus Aspergillus terreus F37 (KX024595) as reducing agent is reported here. The reaction medium employed in the synthesis process was optimized under a narrow range of pH and temperature to attain better yield, controlled size, and more stable of AgNPs. Further, the microbially synthesized AgNPs were studied through UV-vis spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier transform infrared (FT-IR) spectroscopy analyses. The obtained results indicated the formation of high crystalline spherical AgNPs with an average diameter of 45.2±0.5 nm at room temperature (22 ºC). Quantitative analyses indicated that reduction of the Ag+ precursor was promoted at elevated pH due to increased activity of biomolecules in the fungal extract. As a result, the size of the AgNPs decreased with increased pH of the reactions. The optimum conditions for maximum production of small control sized AgNPs (12± 0.5 nm) were pH (10) and temperature (100 ºC). The outcomes of the antifungal activity of different controlled sized AgNPs showed their efficiently to inhibit the mycelial growth of the pathogenic fungus Alternaria solani, the causal agent of tomato early blight disease and reduced their viability in a pH and temperature dependent manner. These findings revealed that the fine tuning of the reaction synthesis parameters, will increase the chance to obtain desired well shaped and small sized AgNPs with potent antifungal activities, may have important applications as new bio-fungicides in controlling various plant diseases caused by fungi.
How to Cite
Mohamed, M., Hussein, H., & Ali, A. (2018). Antifungal activity of different size controlled stable silver nanoparticles biosynthesized by the endophytic fungus Aspergillus terreus. Journal of Phytopathology and Pest Management, 5(2), 88-107. Retrieved from http://ppmj.net/index.php/ppmj/article/view/159
Authors who publish with Journal of Phytopathology and Pest Management agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under the Creative Commons Attribution-Non Commercial License (CC BY-NC). This allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Archives of Agricultural Sciences Journal is an Open Access Journal, and articles published are distributed under the terms of the Creative Commons Attribution-Non Commercial License (CC BY-NC). Readers may copy, distribute, and display the work for non commercial purposes with the proper citation of the original work. However, the journal retains the right to exploit subsidiary rights on behalf of the authors.
- Authors are able to enter into separate, additional contractural arrangements for the non-exclusive distribution of the journal's published version of the work (e.g. post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process with full disclosure to the journal, as it can lead to productive exchanges, as well as earlier and greater citation of published work. Following publication in Archives of Agricultural Sciences Journal, the author should update the repository, and include a citation and link to the published work.
Click here for more information on Licensing policy